Evaluation of in Vitro Embryotoxicity of Dental Monomers by Differentiation of Embryonic Stem Cells into Contracting Cardiac Myocytes

Koichi Imai1, Toru Hayakawa2 and Masaaki Nakamura1
1Department of Biomaterials, Osaka Dental University. Japan
2Department of Dental Biomaterials, Nihon University. School of Dentistry at Matsudo, Japan

AATEX 12(2):171-178, 2007

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3 cells) were examined using the embryonic stem cell test (EST). Three study parameters, the rate of differentiation into contracting cardiomyocytes, the 50% cell viability of ES-D3 and BALB/c 3T3 cells, and the clone A31 (3T3 cells), were used to determine the embryotoxicity. Of the eighteen dental monomers tested, Bis-GMA (6F) and MTYA were classified as weakly embryotoxic, and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, 6-HHMA, MEPC, Phosmer M, BPE-1300, BSNa, EDMABA, GAM, GMR, PTSNa, and QTX were evaluated as nonembryotoxic. However, none of the monomers tested were found to be strongly embryotoxic.
Our results suggest that further intensive studies on the embryotoxicity of dental biomaterials are needed in addition to examination of conventional biological aspects from the perspective of biological safety.

Key words: dental Monomer, embryotoxicity, EST


(AATEX: Altern. Animal Test. EXperiment.: Alternatives to Animal Testing and EXperimentation)