ORIGINAL MANUSCRIPT
Cell Viability Test for Alkalinized Lidocaine using the Three-dimensional Cultured Human Skin Model

Yoshihiro Momota1, Koichi Imai2, Naotaka Kishimoto1, Kazutomo Yamabayashi1 and Junichiro Kotani1

1Department of Anesthesiology, 2Department of Biomaterials, Osaka Dental University, Osaka, Japan

AATEX 13(3):123-126, 2008

Abstract
Tissue damage by lidocaine and alkalinized lidocaine was studied using a three-dimensional cultured skin model. Water for injection, dibasic potassium hydrogen phosphate, and sodium bicarbonate were added to 4% lidocaine, and pH was measured. Dibasic potassium hydrogen phosphate and sodium bicarbonate were added to phosphate buffered saline (PBS) (pH 7.4) and adjusted so that they would be the same as the obtained pH (7.85, 7.90). Each of the test drugs were added to TESTSKINTM (LSE-d) and cultured, and were compared using the MTT method regarding cell viability.
The formazan absorbance decreased in the alkalinized lidocaine compared to PBS and alkalinized PBS. On the other hand, no significant difference was observed in formazan absorbance between lidocaine that was not alkalinized (pH 6.7) and PBS, so it is believed that the alkalinization of lidocaine may have a possibility of decreasing cell viability.

Key words: lidocaine, human skin model, cell viability, Lidocaine

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(AATEX: Altern. Animal Test. EXperiment.: Alternatives to Animal Testing and EXperimentation)