Cardiotoxicity of Anticancer Drug in Chick Embryos

Hiroyuki Miyazaki1, Toshimi Iizuka1, Motohiro Okayasu1, Toshihiro Noda2, Takenori Tamaki1, and Yuji Yoshiyama1

1Division of Community Pharmacy, Center for Clinical Pharmacy and Clinical Sciences,
Kitasato University School of Pharmacy, 1-5-9, Shirokane, Minato-ku, Tokyo, Japan
2Jujin Pharmacy, 8-504, Ichijyodori, Asahikawa, Hokkaido, Japan

AATEX 14(3):954-957, 2009

The cardiac toxicity of arsenic trioxide (ATO) was studied in chick embryos. Fertilized eggs of White Leghorns were incubated and investigated. The chick embryonic heart has been often used in pharmacologic and toxicologic experiments. After ATO at 0.25, 0.5 or 1.0 mg/egg was injected into fertilized eggs, heart rates (HRs) were measured by electrocardiogram. After low dosing of ATO, the heart rate was not different compared with control. However, HRs significantly decreased in a dose- and time-dependent manner (p<0.05). In addition, arrhythmia was produced by high dosing of ATO. These findings indicate that ATO has a marked influence on the heart rate dose- and time-dependently in chick embryos.

Key wards: cardiotoxicity; anticancer drug; arsenic trioxide; chick embryo; electrocardiogram


(AATEX: Altern. Animal Test. EXperiment.: Alternatives to Animal Testing and EXperimentation)