ORIGINAL ARTICLE
The Japanese Ring Study of a Human Cell Line Activation Test (h-CLAT) for Predicting Skin Sensitization Potential (6th Report): A Study for Evaluating Oxidative Hair Dye Sensitization Potential Using h-CLAT

Kenji Okamoto1, Yoshinao Kato2, Nanae Kosaka3, Makoto Mizuno4, Hiroyuki Inaba5, Sakiko Sono6, Takao Ashikaga6, Tsuneaki Nakamura5, Yuko Okamoto4, Hitoshi Sakaguchi3, Masataka Kishi 2, Hirofumi Kuwahara1 and Yasuo Ohno7

1Kanebo Cosmetics Inc., Kanagawa, Japan; 2Nippon Menard Cosmetic Co., Ltd., Aichi, Japan; 3Kao Corporation, Tochigi, Japan; 4KOSÉ Corporation, Tokyo, Japan; 5Lion Corporation, Kanagawa, Japan; 6Shiseido Co., Ltd., Kanagawa, Japan; 7National Institute of Health Sciences, Tokyo, Japan

AATEX 15(2):81-88, 2010

We are conducting a Japanese ring study to develop the human Cell Line Activation Test (h-CLAT). The aim of this study is to confirm whether the h-CLAT can predict skin sensitization for oxidative hair dyes. In addition, we studied the effect of test chemical fluorescence on prediction performance. h-CLATs were independently performed in two laboratories for eight chemicals, yielding good reproducibility between the laboratories. Good concordance was obtained between LLNA results and study chemicals. p-Phenylenediamine, which exhibits fluorescence at around 530 nm, which is in the h-CLAT measurement range, was correctly evaluated as positive by h-CLAT. It was possible to compensate for the influence of fluorescence in CD86/CD54 expression measurements, suggesting that substances exhiviting autofluorescence, such as oxidative hair dyes, can also be evaluated correctly. While a notably high, concentration-dependent CD54 expression was observed with Bandrowski's base, this expression was not observed with p-phenylenediamine, a result which suggests that the sensitization responses of these two dyes may differ. In conclusion, it is suggested that h-CLAT is useful for evaluating the skin sensitization potential of oxidative hair dyes.

key words: h-CLAT, skin sensitization, alternatives, Bandrowskifs base, hair dye

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(AATEX: Altern. Animal Test. EXperiment.: Alternatives to Animal Testing and EXperimentation)