ASSESSMENT OF TUMOR SENSITIVITY TO ANTICANCER AGENTS BASED ON TUMOR GRAFT VASCULARIZATION ON CHICK CHORIOALLANTOIC MEMBRANE (CAM)

HIROYOSHI NINOMIYA1, TOMOH INOMATA1, TSUNERNORI NAKEMURA1, KIKUMI OGIHARA2, TOKIO TSUCHIYA2 AND ISAO NIIZUMA3
1Departments of Laboratory Animal Science and 2Environmental Pathology, Azabu University, Sagamihara-shi 229 Japan and 3Practitioner, Nerima-ku Tokyo 177, Japan .

AATEX 1(2):79-83


Abstract
Tumors were implanted onto CAM where they were grown and the effects of anticancer drugs were examined following their injection into the yolk sac. To assess vascular response, the number of radially arranged capillaries was counted and compared with those in control CAM-preparations. By this method, tumor sensitivity toward selected anticancer agents could be determined more easily and at less expense than by other assay methods for estimating drug sensitivity. Neither does this method require mammals such as nude mice, elaborate animal room facilities or tissue culture systems.


return