Regular article :AATEX 2(1):19-24
A sensitive enzyme immunoassay (EIA) system that detected 0.1 ng of ornithine decarboxylase (ODC) was developed using a monoclonal antibody (MKO-7) raised against mouse kidney ornithine decarboxylase. This EIA system determined ODC in crude extracts of cultured normal human skin cells treated with the carcinogenic promoter, 12-o-tetradecanoylphorbol-13-acetate (TPA). This system using cultured normal human skin cells could thus provide a convenient screening system for unknown promoters.
Key words: human skin cell, TPA, ODC, EIA.