A NEW APPROACH FOR THE DETECTION OF A CARCINOGENIC PROMOTER: ENZYME IMMUNOASSAY FOR ORNITHINE DECARBOXYLASE IN CULTURED NORMAL HUMAN SKIN CELLS

T. FUJII1, C. TADA1, M. YAMAJI1, S. SAKO1, T. MATSUBARA1, M. IWAMI1, T, TAKAGI1, M. YAKIYAMA1, T. OKA2, and K. MIYAMOTO2
1Laboratory of Biological Science, Teikoku Seiyaku Co., Sanbonmatsu, Kagawa and 2Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho, Tokushima, Japan

Regular article :AATEX 2(1):19-24

Abstract
A sensitive enzyme immunoassay (EIA) system that detected 0.1 ng of ornithine decarboxylase (ODC) was developed using a monoclonal antibody (MKO-7) raised against mouse kidney ornithine decarboxylase. This EIA system determined ODC in crude extracts of cultured normal human skin cells treated with the carcinogenic promoter, 12-o-tetradecanoylphorbol-13-acetate (TPA). This system using cultured normal human skin cells could thus provide a convenient screening system for unknown promoters.

Key words: human skin cell, TPA, ODC, EIA.


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