LONG-TERM CYTOTOXICITY TESTING OF THE MEIC CHEMICALS 21-50 BY THE DETERMINATION OF THE PROTEIN CONTENT IN HUMAN EMBRYONIC LUNG CELLS

PAUL J. DIERICKX1 AND BJORN EKWALL2
1lnstituut voor Hygiene en Epidemiologie, Afdeling Toxikologie, Wytsmanstraat 14, B-1050 Brussel, Belgium; 2Department of Toxicology, University of Uppsala, Biomedical Centre, Box S44, S-S71 24 Uppsala, Sweden

Regular article :AATEX 2(1):33-36

Abstract
Within the framework of the MEIC (the Multicenter Evaluation of In Vitro Cytotoxicity) programme the long-term cytotoxicity of the MEIC chemicals 21-50 was investigated on MRC-5 cells. After 6 weeks treatment the PI50 was measured, which is the concentration (mM) of test compound required to induce at 50% reduction in total cell protein content. The results were compared with the acute PI50, measured after 24 hours in Hep G2 cells. The long-term PI50, values are lower than the acute ones. Warfarin was ten times less toxic in the long-term experiments. For the whole series of chemicals a good correlation between the two sets of data was observed (r=0.96), with the exception of digoxin and warfarin. As with other preliminary MEIC validation results, this study indicates that a good alternative cytotoxicity test may be found for the prediction human long-term toxicity.

Key words: MEIC, MRC-5 cells, long-term cytotoxicity.


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