PROTECTIVE EFFECTS OF 2,3--DIMERCAPTO-1-PROPANOL ON BIS (TRIBUYULTIN) OXIDE-INDUCED CYTOTOXICITY IN ISOLATED RAT HEPATOCUTES

N. SUSA, S. UENO, and Y. FURUKAWA
Department of Veterinary Public Health, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034, Japan


Regular Article :AATEX 3(3):106-110

Abstract
The effects of 2, 3-dimercapto-1-propanol (BAL) on bis (tributyltin) oxide (TBTO)-mediated cell injury were studied using isolated rat hepatocytes. The cells (1x106 cells/ml) were incubated with or without TBTO alone, or with both TBTO and BAL at 37C for 60 min. Simultaneous application of 100 FLM BAL and 100 FLM TBTO reduced the rate of cell injury as evaluated by lactate dehydrogenase leakage and cellular tin content in comparison to TBTO alone. BAL also prevented the stimulation of lipid peroxidation as measured by the production of malonedialdehyde and protected against the depletion of levels of antioxidants such as glutathione (GSH), vitamin C, and vitamin E, and antioxidant enzyme levels such as glutathione reductase and superoxide dismutase induced by TBTO. The results obtained in the present study revealed that BAL is able to prevent TBTO-induced cytotoxicity and suggest that this protective effect may be related to a reduction of the cellular tin content.


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