Original paper :AATEX3(3):111-120
To facilitate development of an alternative test to predict Draize rabbit eye irritation scores (Draize eye score), we investigated optimal conditions for cytotoxicity testing using cultured mammalian cells. Different cytotoxic markers, various cells, treatment periods and serum concentrations in culture medium were assayed using eight surfactants as test agents.
Good correlations with in vivo results were gained with exposure periods of 24 or 48 hours. Little difference between cytotoxic markers was found exception of the lactate dehydrogenase (LDH) assay. The serum concentration in the medium exerted an effect regardless of the primary cell or cell line, cytotoxicity being more pronounced in cells cultured in serum free medium. However, the correlation with Draize eye score was lower for serum.
Key words: eye irritation, cytotoxicity, in vitro.