Cause of low embryogenesis-promoting ability of bovine and swine sera for 9.5-day rat embryo in culture

Miyuki Katoh, Reiko Kimura and Ryujiro Shoji
Department of Embryology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-cho, Kasugai, Aichi 480-0392, Japan.

Correspondence: Miyuki Katoh, Department of Embryology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-cho, Kasugai, Aichi 480-0392, Japan
Tel: 0568-88-0811, Fax: 0568-88-0829

Running Title: Embryogenesis-promoting ability of bovine and swine sera in rat embryo

Original paper :AATEX 6(1):65-71,1999
Abstract
Were bovine and swine sera to have the same embryogenesis-promoting ability as rat serum, they might be able to replace rat serum as a medium for whole rat embryo culture, given their availability at any butchery. When 9.5-day rat embryos were cultured in 50% and 100% of bovine and swine sera, respectively, the embryogenesis-promoting abilities were compared with those of rat serum. Development of rat embryos cultured in 50% and 100% of these sera was significantly poorer than that of embryos cultured in 50% rat sera. Therefore, these sera probably have low embryogenesis-promoting ability for 9.5-day rat embryos in culture. Furthermore, the bovine and swine sera contained a 180 kDa serum component in place of the 190 kDa component in rat serum, and DEAE-eluate in these sera also contained most of the 180 kDa serum component. In addition, development of 9.5-day rat embryos that were cultured in serum-free medium, containing rat transferrin, supplemented with DEAE-eluate in these sera, also was poorer than that of embryos cultured in medium with rat serum. The difference in presence of the defined serum components seemed to reflect the limited embryogenesis-promoting abilities in these sera

Keywords: whole rat-embryo culture, culture medium, domestic-animal's sera, rodent sera


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