Color Doppler Echocardiograms Can Measure Hemodynamic Changes of Cardiovascular Systems in Chick Embryos

Takashi Sugiyama1, Kazuru Saito2, Hideyo Shimada1 and Kanji Tshuchimoto1,2
1Division of Pathophysiology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University, 9-1, Shirokane 5-chome, Minato-ku, Tokyo 108-8641, Japan
2Kitasato Institute Hospital, 9-1, Shirokane 5-chome, Minato-ku, Tokyo 108-8642, Japan

Correspondence: Takashi Sugiyama, Ph.D., Division of Pathophysiology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University

9-1, Shirokane 5-chome, Minato-ku, Tokyo 108-8641, Japan.
Tel: +81-3-3444-6161 ext 3231
Fax:+81-3-3446-9036
E-mail: sugiyamat @ platinum.pharm.kitasato-u.ac.jp

Original paper :AATEX 7(2,3):59-66,2001

Abstract
The aim of the present study was to evaluate the hemodynamic changes of chick embryos by using color Doppler echocardiography, which is one of the essential tools used to determine cardiovascular abnormalities in humans. Fertylisin (FTS), which induces cardiovascular abnormalities in rodents, was injected into the air sac of fertile eggs of White Leghorn chickens on the 2nd day of incubation and the chick embryos were observed grossly and physiologically on the 18th day of incubation. A high frequency of cardiovascular abnormalities including double aortic arch etc. was observed in the FTS-treated chick embryos . Color Doppler flow imaging echocardiograms and first Fourier transformation (FFT) patterns of the heart and aorta of FTS- or calmelose (control)-treated chick embryos were recorded on the 18th day of incubation by using echocardiography with 5 MHz transducer. The values of maximum velocity (MV), flow integration (FI) and acceleration time (AT) obtained from the FFT patterns in FTS-treated chick embryos were decreased compared with those of the control embryos, but the heart rate was increased. These findings suggest that color Doppler echocardiography could be applicable to evaluate the anatomical and physiological abnormalities of chick embryonic cardiovascular systems.

Keywords: chick embryo, Doppler echocardiography, cardiovascular anomaly, fertylisinIntroduction


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