One Trial of the Development of Image Analyzing Method of Cardiomyocytes Derived from ES Cells as Alternative Method of Cardiotoxicity Test Based on an Electrocardiogram -Analysis of Chronotropic and the Inotropic Action-

Sumiko Kawai1, Yasuhiro Takagi1, Kyoko Shioya2, and Tsutomu Kurosawa1

1Department of Laboratory Animal Medicine, Osaka University Medical School, Osaka, Japan
2Laboratory of Animal Experiment and Medicine Management,
National Cerebral and Cardiovascular Center, Osaka, Japan

AATEX 17(1):9-12, 2012

Beating images of the cardiomyocyte derived from an embryonic stem cell were analyzed using our image analytical method to analyze the image recorded by a video camera. The pulsatile frequency and intensity of the beating cardiomyocyte were inhibited after exposure to Diphenylhydantoin (DPH). Therefore, it became apparent that we could examine the presence or absence of chronotropic action and inotropic action to cardiac muscle cells only by on in vitro test. These results suggested that our method was regarded as a new practical cardiotoxicity test method alternative to the animal experiment.

key words: Cardiomyocytes, ES cells, chronotropic actions, inotropic actions, Image


(AATEX: Altern. Animal Test. EXperiment.: Alternatives to Animal Testing and EXperimentation)