An Attempt to in Vitro Embryotoxicity Test using Mouse ES Cells and Human Hepatocytes

Koichi Imai1, Akito Tanoue2, Kazuaki Nakamura2, Yoshitomo Honda3, and Kazuhiko Suese4

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
3Institute of Dental Research, Osaka Dental University, Osaka, Japan
4Osaka Dental University, School of Dental Technician and Hygienist, Osaka, Japan

AATEX 18(1):33-38, 2013

On evaluating the effects of dental biomaterials on embryotoxicity in humans, the effects of human metabolic activation factors cannot be ignored. However, the routine the Embryonic Stem Cell Test (EST) method does not reflect the effects of metabolic activation in humans, because of the use of mouse ES-D3 cells. Thus, a TEST LIVERTM-human (Toyobo, Osaka) culture with teratogenic thalidomide was exposed to mouse ES-D3 cells to evaluate cell differentiation of contracting myocardial cells. Also, standard reagents for atomic absorption spectroscopy, i.e., component elements of dental alloys (Ag, Cu, Pd, Sn and Zn), were used. As a result, thalidomide, Ag, and Sb significantly reduced the contraction rate compared with the control group. A preliminary culture using TEST LIVERTM-human is likely to facilitate evaluation of the effects of human metabolism in mouse ES cells.

key words: embryotoxicity, thalidomide, ES cells, human hepatocytes, metal ions


(AATEX: Altern. Animal Test. EXperiment.: Alternatives to Animal Testing and EXperimentation)