The distributions of intracellular pH, Na+, K+, Mg2+ and Ca2+ concentration is measured using various type fluorescent indicator dyes. The measurement of these intracellular ion distributions performed by a ratio imaging method. The equipment constructed with a fluorescence microscope and digital image processor.
Many type indicator dyes had the property of collection in nucleus, but only Na+ dye had a collection in cytoplasm. The intracellular pH, K+ and Ca2+ distribution was observed homogeneously distribution. However, the intracellular Na+ and Mg2+ shows the concentration in cytoplasm and nucleus were clearly different.