METHODS FOR EVALUATING TERATOGENIC ACTIVITIES OF CHEMICALS AND SERUM FLUIDS USING MICROMASS CULTURE SYSTEM

TOSHIE TSUCHIYA, YOHIAKI IKARASHI AND AKITADA NAKAMURA
Division of Medical Devices, National Institute of Hygienic Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158, Japan

AATEX 1(3):165-171

Abstract
Mouse or rat embryonic midbrain (MB) and limb bud (LB) cells were prepared from day 10-13 embryos and cultured as micromass cell islands for 5-7 days. Differentiation was determined by the number of stainable foci of differentiated ceils, and the concentration at which each compound inhibited the formation of differentiated foci by 50% of the control value (IC50) was estimated for evaluating the teratogenic potentials of compounds.
We apply the micromass teratogen test to the studies on the causes of TBZ-induced teratogenesis and species differences in the ETU-induced teratogenesis.


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