Toxic Interactions between Amitriptyline and Disopyramide in Chick Embryos

Yuji Yoshiyama1, Takashi Sugiyama2, and Motoko Kanke1
1Division of Clinical Pharmacy, Kyoritsu University of Pharmacy, 1-5-30, Shibakoen, Minato-ku, Tokyo 105-8512, Japan
2Division ofPathophysiology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan

Original paper :AATEX 10(1):18-23
The toxic interaction between amitriptyline and disopyramide were studied in chick embryos. Fertilized eggs of White Leghorns were incubated and investigated. Amitriptyline with and without disopyramide was injected into the air sac of a fertilized egg on the 16 th day of incubation. Electrocardiograms were recorded 0 to 60 min after the injection. After each drug injection alone, the heart rate was not different compared with control. However, the heart rate was significantly decreased by combination with amitriptyline and disopyramide. In addition, arrhythmia was produced by amitriptyline with disopyramide. These findings indicate that the interaction between amitriptyline and disopyramide has a marked influence on the heart rate in chick embryos.

Key words: toxic interaction, amitriptyline, disopyramide, chick embryo, electrocardiogram