Experimental Study on a Novel Chemical Application Procedure for in Vitro Skin Corrosivity Testing Using the Vitrolife-SkinTM Human Skin Model

Noriyuki Morikawa1, Katsuyasu Morota1, Masakazu Suzuki1, Hajime Kojima2, Satoru Nakata2 and Hiroaki Konishi2
1 Division of Research & Development, GUNZE Ltd., 1 Ishiburo, Inokura-shinmachi, Ayabe, Kyoto 623-8512, Japan
2 Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-ku, Nagoya 451-0071, Japan

AATEX 11(1):68-78, 2005

During the last decade, ECVAM has supported formal validation studies on in vitro tests for predicting skin corrosivity, which included two of the in vitro tests employed human skin models, EPISKINTM and EpiDermTM. When skin models are used to evaluate skin irritancy and corrosivity, it is important that suitable chemical application procedures are utilized. We used several chemicals and evaluated their skin corrosivity using in vitro tests, evaluations using ECVAM experimental protocols and post-incubation methods that we have originally developed to predict skin irritancy, and compared the results. Vitrolife-Skin showed basic utility for corrosivity testing by both methods and it is possible to confidently predict skin corrosivity, provided that the appropriate chemical application procedures are used. In the future, human skin models will become very useful for purposes of predicting in vivo skin corrosivity of chemicals and for studying the mechanistic aspects of such corrosivity.

Key words:human skin model, skin corrosivity, Vitrolife-Skin, EpiDerm, ECVAM