CYTOTOXICITY TEST OF ETHANOL USING VARIOUS HUMAN HEPATOMA AND OTHER CELL LINES

Y. GU1, M. MIYAZAKI1, T. OHNO2, AND M. NAMBA1,3
1Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata, Okayama 700, Japan; 2Riken Cell Bank, Koyadai, Tsukaba Science City, Japan

Regular article :AATEX 2(1):1-5

Abstract
The cytotoxic effects of ethanol on the colony-forming capacity of six human hepatoma cell lines, Hep G2, Hep 3B, HLE, HuH-6, HuH-7, and PLC/PRF/5, and two other human cell lines, T24 (bladder cancer) and KMST-6 (human fibroblasts immortalized with Co-60 gamma rays), were investigated. A closed system was used for the cytotoxicity assay to prevent the rapid evaporation of ethanol. The hepatoma cell lines did not show significant differences in their sensitivity to the cytotoxic effects of ethanol; dose-dependent cytotoxicity was observed. The hepatoma cell lines, Hep 3B, HLE, and PLC/PRF/5, were suitable for cytotoxicity assay, being easy to culture and to prepare single cells by trypsinization and forming distinctly countable colonies; Hep G2, HuH-6, and HuH-7 cells were not useful, as they were difficult to culture and formed colonies which were hard to count. T24 cells were insensitive to ethanol cytotoxicity up to an ethanol concentration of 1%, while KMST-6 cells were as sensitive to ethanol as the hepatoma cell lines. These results indicate that ethanol cytotoxicity can be assessed in cells other than liver cells in culture.


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