CYTOTOXICITY TEST OF METHANOL USING VARIOUS HUMAN HEPATOMA CELL LINES

Y. GU1, T. TOKIWA1, K. KINO2, T. OHNO3 AND M. NAMBA1
1Department of Cell Biology, Institute of Molecular and Cellular Biology, 2Department of Surgery, Okayama University Medical School, 2-5-1 Shikata, Okayama 700, Japan; 3Riken Cell Bank, Koyodai, Tsukaba Science City, Japan

Regular article AATEX 2(3):105-108

Abstract
The cytotoxic effects of methanol on the colony-forming capacity of six human hepatoma cell lines, Hep G2, Hep 3B, HLE, HuH-6, HuH-7 and PLC/PRF/5, were investigated. A closed system was used for the cytotoxicity assay, to prevent the rapid evaporation of methanol. The lethal dose of methanol for 50% cell death lay between 1% and 1.5%. Among the six hepatoma cell lines, Hep 3B and HuH-6 lines were a little more sensitive to methanol cytotoxicity than the other cell lines. Interestingly, alcohol dehydrogenase activity was inducible only in HuH-6 line after 24 hour exposure to 0.5% ethanol, though its activity was about one-half of that of the rat liver tissue.


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