Regular article AATEX 2(4):203-214
Surfactant induced oral mucosal irritation and desquamation were assessed by the two-dimensional in vitro test which included neutral red assay, indirect immunostaining for fibronectin and tubulin, western blot for tubulin, and scanning electron microscopic (SEM) evaluation on normal human embryo kerotinocyte monolayers. Mucosal desquamation was also assessed by the three-dimensional in vitro test which included light microscopic (LM) and SEM evaluations of a three-dimensional culture of normal human gingival keratinocytes and fibroblasts treated with surfactants. Neutral red assay, and SEM evaluation in the two-dimensional in vitro test gave an index of the oral mucosal irritation. And, indirect immunostaining in the two-dimensional in vitro test, and LM and SEM evaluations in the three-dimensional in vitro test provided an index of desquamation. Benzalkonium chloride treatment resulted in mainly inflammation, while both sodium lauryl sulfate and sodium lauroyl sarcosinate treatments caused mainly desquamation. Tween 20 and Sucrose fatty acid ester did not produce any effects.
It seems that the evaluation of intra- and extra-cellular matrix is simple in the in vitro alternative method for oral mucosal desquamation.