Regular article AATEX 2(4):215-223
Based on the hypothesis that irritation is due to damage of the cellular plasma membrane and cellular proteins, we designed an in vitro battery test system for predicting eye irritancy. The system consists of 3 tests, a hemoglobin denaturation (HDR) test to evaluate the protein denaturation factor, and tests of red blood cell (RBC) Iysis and liposome Iysis to evaluate the cellular plasma membrane destruction factor. Multi-regression analysis of the data obtained yielded equations for predicting Draize eye irritation scores. A combination of HDR and liposome tests gave the highest correlation to corneal Draize score (r=0.922), while combination of HDR and RBC tests gave the best correlation to total Draize score (r=0.941). These correlations are sufficiently good that this in vitro battery test system should represent a practical alternative to the in vivo Draize test for predicting eye irritancy of chemicals.