Regular Article :AATEX 3(4):168-174
The MATREXTM system developed by Organogenesis, INC, has a three-dimensional structure of collogen gel and human fibroblasts. We evaluated this method in three laboratories as an alternative method to the Draize rabbit eye irritation test using coded nine surfactants and isotonic sodium chloride solution. Results were:
The mean coefficient of variance among the three laboratories for the ten substances was 0.220; The correlation coefficient between in vitro EC50 values on inhibit of MIT reduction in MATREXTM and the Draize scores (maximum average Draize total score: MAS) was 0.633.
The advantage of the MATREXTM is considered to be the ability to apply water insoluble substances It was not possible to assess this potential advantage in this study since all test substances done at this phase of validation were water soluble, however this will be checked during the second phase of validation. when insoluble substances will be evaluated.
Key words: validation study, Draize eye irritation test, alternatives, in vitro, MATREXTM, surfactant.